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Embedding Skin Tissue

Although most histotechnologists consider skin embedding a challenge (or frustration), the process can be made simple by standardizing the methods to complete the task. Thorough training and consistency in embedding are the keys to optimal microscopic slides. Each step in tissue processing is critical and dependent on the previous step; however, embedding skin in the proper orientation truly has the most impact on the diagnostic outcome. In blocks that contain improperly embedded skin, the areas of diagnostic importance MAY BE CUT AWAY and a diagnosis may not be possible. Re-sampling of skin samples is not typically performed since the area of interest is isolated and sampled once. Histotechnologists get only one shot to get it right. The following pages represent proper embedding techniques for skin samples.