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When to Suspect an Autoantibody

Autoantibody (Cold or Warm)

Suspect an autoantibody if:

  • All panel and screen cells are positive and the autocontrol (AC) is positive.
  • Reaction strength in all the cells are usually the same.

Cold autoantibody: strongest reactions occur at immediate spin phase. Reactions may weaken in strength at 37°C and AHG.

Warm autoantibody: strongest reaction occurs at AHG. Reactions may be seen at 37°C and usually not seen at immediate spin.