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A histotechnology student manually deparaffinizes and performs hematoxylin and eosin (H&E) stains on a dozen tissue slides, only to discover that all of the slides were blank. Not a trace of tissue remained on the slides after the staining was completed. The puzzled student went to his supervisor for assistance. What question(s) could the supervisor ask to determine the source of the problem? (Choose all that apply.)

More than one answer is correct. Please select all correct answers