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The page below is a sample from the LabCE course Reading Gram Stained Smears From Cultures. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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Examining the Smear

Gram stained direct smears are examined using the oil immersion objective of the microscope (total magnification =1000x). Use the lower power objective to focus on the field, then switch to the 1000x oil immersion objective to examine the appearance of the bacteria present.