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Amplified Nucleic Acid Tests

Amplification Method


Use of Thermal Cycling (Thermocycling)

Polymerase Chain Reaction (PCR)Target amplification using DNA polymerase


Ligase Chain Reaction (LCR)Target amplification using DNA ligase Yes
Transcription- based or Transcription-mediated amplification(TMA)

Target amplification using reverse transcriptase and RNA polymerase

Strand Displacement (SDA)Target amplification using DNA polymerase that continuously displaces strands of DNA containing the target sequence
Branched DNA (bDNA)Signal amplification using alkaline phosphataseNo
Loop Mediated (LAMP)

Target amplification of multiple DNA sequences in a loop pattern using DNA polymerase

Nucleic acid sequence based (NASBA)Target amplification using 3 enzymesNo
Q-beta Replicase

Probe amplification- The concentration of an RNA probe increases if the target is present