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The page below is a sample from the LabCE course Fundamentals of Molecular Diagnostics. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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Amplified Nucleic Acid Tests

Amplification Method
Use of Thermal Cycling (Thermocycling)
Polymerase Chain Reaction (PCR)Target amplification using DNA polymerase
Ligase Chain Reaction (LCR)Amplification of a short DNA probe using DNA ligase Yes
Transcription- based or Transcription-mediated amplification(TMA)
Target amplification using reverse transcriptase and RNA polymerase
Strand Displacement (SDA)Target amplification using DNA polymerase that continuously displaces strands of DNA containing the target sequence
Branched DNA (bDNA)Signal amplification using alkaline phosphataseNo
Loop Mediated (LAMP)
Target amplification of multiple DNA sequences in a loop pattern using DNA polymerase
Nucleic acid sequence based (NASBA)Target amplification using 3 enzymesNo
Q-beta Replicase
Probe amplification- The concentration of an RNA probe increases if the target is present