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The page below is a sample from the LabCE course Fundamentals of Molecular Diagnostics. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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Methodologies can be classified as to the target of interest, detection techniques, or the necessity for amplification.
Targets of interest can include:
  • DNA
  • RNA
  • Bacteria
  • Fungus
  • Virus (see bacteriphage image to the right)
  • Antibody
  • Antigen
Detection techniques can include:
  • Chemiluminescence
  • Electrophoresis
  • Enzyme
  • Fluorescence
  • Hybridization
  • Radioactivity
Amplification is not necessary in direct techniques.