Home Products Most Popular Contact
No items in your cart.
The page below is a sample from the LabCE course Technical Competence in Paraffin-Based Fluorescence In Situ Hybridization (FISH). Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

Learn more about Technical Competence in Paraffin-Based Fluorescence In Situ Hybridization (FISH) (online CE course) »
How to Subscribe
Histology CE Package$65 Add to cart
Individual course$20 Add to cart

Patient Slide

Patient slides are enumerated following assessment and enumeration of the quality control (QC) slide. The patient slides should be assessed for the same parameters as the QC slide before enumeration is performed. Enumeration entails documenting the number of signals for each probe on an excel spreadsheet and then calculating the average of each probe. Normally, 60 TUMOR cells are counted. For some probes, as few as 20 cells are counted while for other probes as many as 200 cells are counted.
In the image, there are 3 green centromere signals, indicating aneuploidy of chromosome 10. There is 1 orange PTEN signal, which indicates a relative loss of PTEN. This means that the PTEN gene was lost on 2 of the chromosomes.