This is an example of a routine processing protocol (for large tissue sections) with a closed-system processor, using standard processing reagents and also pressure/vacuum. Compare the tables on the following pages and note how many hours it takes to process routine surgical tissue versus smaller biopsies, and also fatty tissue.
10% neutral buffered formalin (NBF) | 90 |
10% NBF | 90 |
70% alcohol | 30 |
95% alcohol | 60 |
95% alcohol | 60 |
100% alcohol | 60 |
100% alcohol | 60 |
100% alcohol | 60 |
Xylene | 60 |
Xylene | 60 |
Paraffin | 40 |
Paraffin | 40 |
Paraffin | 40 |
Paraffin | 40 |