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The page below is a sample from the LabCE course Tissue Processing in Histology. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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Routine Tissue Processing

This is an example of a routine processing protocol (for large tissue sections) with a closed-system processor, using standard processing reagents and also pressure/vacuum. Compare the tables on the following pages and note how many hours it takes to process routine surgical tissue versus smaller biopsies, and also fatty tissue.
ReagentTime (minutes)
10% neutral buffered formalin (NBF)90
10% NBF90
70% alcohol30
95% alcohol60
95% alcohol60
100% alcohol60
100% alcohol60
100% alcohol 60
Xylene60
Xylene60
Paraffin40
Paraffin40
Paraffin40
Paraffin40