RSV is a single-stranded RNA virus of the Paramyxoviridae family and includes respiratory viruses such as measles and mumps. The F proteins on the surface of the virus cause the cell membranes on nearby cells to merge, forming multinucleate cells, known as syncytia. The cell cytoplasm is the target area for demonstrating RSV.
The author's source for goat anti-human RSV polyclonal antibody requires proteolytic enzyme pretreatment (0.25% pepsin). Anti-goat polymer detection systems are available, but are not as common as labeled polymer detection systems used for detecting mouse and rabbit primary antibodies. Biotin/streptavidin detection systems work well for demonstrating RSV.
The IHC images show pulmonary infection of RSV within the cytoplasm of the infected cells, demonstrated with goat polyclonal antibody, using a horesradish peroxidase (HRP) detection system and DAB chromogen.
