The page below is a sample from the LabCE course Erythrocyte Inclusions. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

Learn more about Erythrocyte Inclusions (online CE course) »
How to Subscribe
MLS & MLT Comprehensive CE Package
Includes 94 CE courses, most popular
$95 Add to cart
Pick Your Courses
Up to 8 CE hours
$50 Add to cart
Individual course$20 Add to cart

Siderotic Granules

To verify that red cell inclusions contain iron, it is necessary to use an iron stain, such as Prussian blue. The iron-containing granules are called siderotic granules. Pappenheimer bodies are siderotic granules that are visible on a Wright-stained smear. All Pappenheimer bodies are Prussian blue positive, but not all siderotic granules are visible on Wright-stained smears as Pappenheimer bodies (remember that Wright or Wright-Giemsa stain does not stain the iron, but rather the protein matrix that contains the iron).
A mature erythrocyte containing siderotic granules is referred to as a siderocyte, while an immature (nucleated) erythrocyte containing siderotic granules is known as a sideroblast.