Sample type required: Deparaffinized and rehydrated tissue section (6 μ) on positively (+) charged slides
Preferred fixative: 10% neutral buffered formalin (NBF)
Control: Normal spinal cord
|0.5% cresyl echt violet||2 minutes|
|Distilled water||3 changes|
|70% alcohol||Differentiate until background is colorless||Check microscopically for endpoint|
Post staining procedure: Tissue section should be dehydrated with 95% and absolute alcohols. Follow with two changes of xylene and then coverslip.
- Nissl substance - Dark blue to purple