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The page below is a sample from the LabCE course Histology Special Stains: Nervous Tissue. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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Cresyl Echt Violet Staining - Chemistry

Cresyl echt violet is used to stain for neuronal cell bodies and processes. Nissl substance has a high ribonucleic acid (RNA) content, making it very basophilic. Cresyl echt violet is a basic aniline dye used to selectively stain Nissl by immersing tissue sections in the staining solution and then differentiating in alcohol until the background is colorless.