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The page below is a sample from the LabCE course Molecular Methods in Clinical Microbiology. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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The Key Benefits: Improved Sensitivity of Detection, continued

Some organisms are present in infections in very low numbers, which may be undetectable with direct staining methods. These organisms may also prove to be difficult to recover with currently available culture methods.

Although non-culture antigen methods have been developed to address some of these difficulties (examples include direct fluorescent antibody (DFA) and enzyme immunoassay (EIA) methods), the sensitivity of these methods has not always been desirable.

Molecular methods offer the prospect of:

  • Detecting nonviable organisms that did not survive transport
  • Detecting organisms difficult/impossible to cultivate
  • Detecting organisms present in low numbers
  • Providing better detection capability than other non-culture methods