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Sexually Transmitted Bacterial Infections (by ASCLS)
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Rapid Plasma Reagin Test
Detects antibodies to reagin: IgG and IgM antibodies directed against a lipoidal antigen resulting from interaction of
T. pallidum
and host tissues
Performed on unheated plasma or serum, not CSF
If antibodies are present, they combine with the lipid particles of the antigen, causing them to agglutinate.
The charcoal particles coagglutinate with the antibodies and show up as black clumps against the white card
#2, 3, 4, 6, 8, and 10 spots on the photograph of an RPR card are reactive and should be serially diluted and retested to report a titer
Rough looking reactions, such as #6, could indicate prozone effect
After dilution, if prozone is present, a strong reaction will be seen in higher dilutions
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