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The page below is a sample from the LabCE course Chemical Screening of Urine by Reagent Strip. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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Urobilinogen Reaction

The test for urobilinogen is based on the Ehrlich Aldehyde Reaction. P-dimethylaminobenzaldehyde in an acid medium with a color enhancer reacts with urobilinogen to form a pink-red color.
The urine reagent strip reactivity increases with increasing temperature. The optimum temperature for testing is 22-26°C.
A freshly voided sample should be tested to ensure optimal results.