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The page below is a sample from the LabCE course Chemical Screening of Urine by Reagent Strip. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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Nitrite Test Sensitivity

Any degree of uniform pink color should be considered positive, suggesting the presence of 105 or more organisms/mL. Detection of low levels of nitrites may be enhanced by placing the activated test strip against a white background.
Note that color development is NOT proportional to the number of bacteria present.
The test is specific for nitrite and does not react with any other substances normally present in urine. Negative results do not necessarily rule out a urinary tract bacterial infection. An infection may be caused by bacteria that do not reduce nitrate.