The test for glucose involves a double-sequential enzyme reaction. In the first reaction, glucose oxidase catalyzes the oxidation of glucose to gluconic acid and hydrogen peroxide. Then, the peroxidase in the glucose pad catalyzes the oxidation of a chromogen by the hydrogen peroxide to form a colored product.
This method is specific for glucose; it does not react with other reducing substances (eg, lactose, fructose, and galactose).
NOTE: The urine specimen should be analyzed while at room temperature for these enzyme reactions to occur properly.