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Laboratory Diagnosis of Neisseria gonorrhoeae: Presumptive

  • Gram-negative intracellular diplococci in a urethral smear (males only)
  • Culture

    • Growth on enriched chocolates agar with isovitalex and on selective media such as Modified Thayer Martin that contains antimicrobials to inhibit normal flora
    • Grows at 35 to 37 degrees C under increased carbon dioxide
    • Usually visible on media at 24 hours as small, tan colonies
    • Colony morphology:
      • Freshly isolated strains exhibit at least two of four colony forms: T1, T2, T3, and T4
      • T1 colonies predominate in initial isolation
      • T3 colonies predominate in subcultures
    • Presumptive identification: typical colony formation on selective media with oxidase positive, gram negative diplococci
PHIL

http://www.cdc.gov/std/Gonorrhea/lab/Ngon.htm