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The page below is a sample from the LabCE course Theoretical and Practical Aspects of Routine H&E Staining. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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Dark Precipitate On The Slide

If the hematoxylin is used beyond its' expiration date or has been improperly stored (excessive heating or freezing) it will show signs of deterioration in the form of black precipitate scattered throughout the section.
Proper storage and attention to expiration dates will prevent this problem. Manufacturer's of H&E provide guidelines for storage requirements and shelf-life.