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The page below is a sample from the LabCE course Theoretical and Practical Aspects of Routine H&E Staining. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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White Patches on Slides After Deparaffinization Step

Sections should appear clear after the deparaffinization step. If white patches are present, this in an indication that the slides were not completely dried and/or deparaffinized.

Possible Causes:

  • Insufficient drying time
  • Insufficient time in xylene


  • Return the slides to the oven or place them in 100% alcohol
  • 2-3 changes of xylene over a period of 10 minutes should be sufficient time to remove all of the paraffin