Staining Time Comparisons

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The page below is a sample from the LabCE course Theoretical and Practical Aspects of Routine H&E Staining (retired 6/6/2018). Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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Staining Time Comparisons

Hematoxylin Staining Time in Minutes
Gill's 5-15
Delafield's 15-20

Mayer's (regressive)

Mayer's (progressive)

5-10

10-20

Harris's 5-15
Ehrlich's 20-45
Heidenhain's 60+
Weigert's 15-30