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The page below is a sample from the LabCE course Theoretical and Practical Aspects of Routine H&E Staining. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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Eosin Differentiation

Differentiation of the eosin staining occurs in the subsequent water wash and continues as the tissue goes through the dehydration alcohols.

The desired result should yield the following three shades of pink:

  • Orange-pink: Red blood cells
  • Light pink: Connective tissue
  • Dark pink: Muscle fibers

The cytoplasm of the cells will contain varying shades of pink.