Denaturaton

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Denaturaton

Denaturation is the process that causes the paired strands of DNA to separate into individual single strands.

The reaction mixture is heated to approximately 95°C for 20-30 seconds in order to promote DNA denaturation. At this point, the hydrogen bonds within the DNA break, resulting in single-stranded DNA molecules. The Taq polymerase is still able to survive at this temperature, but will not activate.