Home Products Most Popular Contact
No items in your cart.
The page below is a sample from the LabCE course Real-Time PCR. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

Learn more about Real-Time PCR (online CE course) »
How to Subscribe
MLS & MLT Comprehensive CE Package
Includes 106 CE courses, most popular
$95 Add to cart
Pick Your Courses
Up to 8 CE hours
$50 Add to cart
Individual course$20 Add to cart

Stages of Real-Time PCR

The PCR process can generally be divided into three stages and visualized on a graph.

The first stage is the exponential amplification where the doubling of the DNA product will occur with each cycle. At this stage there is an excess of reagents and a limited number of templates, so product renaturation does not compete with primer binding.

The second stage is the leveling off stage in which the reaction slows down due to the loss of activity of the DNA polymerase and the consumption of reagents. At this point the product renaturation is competing with primer binding.

The third stage is the plateau stage in which no more products accumulate due to a complete exhaustion of dNTPs and reagents.