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Interpreting Initial Serologic Tests

In cases like those of Patient A.D. two main serologic questions need to be answered:

  1. Are the reactions due to passive anti-D from RhIg or due to active (immune) anti-D?
  2. Are there other antibodies that need to be excluded

Unfortunately, since there is no way to definitively differentiate immune from passive anti-D, certain assumptions are usually made.

Most laboratories first confirm that, as expected, only anti-D is being detected.