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The page below is a sample from the LabCE course Rh-Negative Female with Anti-D at Delivery: A Case Study (retired 6/11/2018). Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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Interpreting Initial Serologic Tests

In cases like those of Patient A.D. two main serologic questions need to be answered:
  1. Are the reactions due to passive anti-D from RhIG or due to active (immune) anti-D?
  2. Are there other antibodies that need to be excluded?
Unfortunately, since there is no way to definitively differentiate immune from passive anti-D, certain assumptions are usually made.
Most laboratories first confirm that, as expected, only anti-D is being detected.