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The page below is a sample from the LabCE course Hemolytic Disease of the Fetus and Newborn. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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Quantifying FMH

When a screening test such as the rosette test indicates a significant FMH, a quantitative test is required to determine the dose of RhIG required. Tests include
  • Kleihauer-Betke acid elution test (KB test);
  • Flow cytometry;
  • Other techniques that are less commonly used, eg, fluorescence microscopy and enzyme-linked antiglobulin test.
The KB and flow cytometry methods are briefly reviewed on the following pages.