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The page below is a sample from the LabCE course Department of Transportation (DOT) Federally Regulated Urine Specimen Collection Training. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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Shy Bladder Procedures, continued

If the donor has not provided a sufficient specimen within three hours of the first unsuccessful attempt to provide the specimen, the collector must discontinue the collection, note the fact on the “Remarks” line of the CCF (Step 2), and immediately notify the Designated Employee Representative (DER).
Note: The collector should maintain a record in the "Remarks" line on the CCF of the time of each attempt, whether there was any specimen provided or the quantity of specimen provided, and the amount of fluids that the donor was given to drink. During the waiting period that the donor can consume fluids, the donor must be monitored to ensure the continued integrity of the test. While there is no requirement for the collector to do so, it is a good practice for the collector to inform the donor that he or she is not permitted to leave the collection site and that doing so could lead an employer to determine that a refusal occurred.
The collector then sends Copy 2 of the CCF to the MRO and Copy 4 to the DER. This is done even if the donor did not provide any specimen in order to notify the MRO and the employer of the problem. The collector must send or fax these copies to the MRO and DER within 24 hours or the next business day.