The page below is a sample from the LabCE course Department of Transportation (DOT) Federally Regulated Urine Specimen Collection Training. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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Collector Applies Tamper-Evident Seals

After dispensing the urine specimen into the specimen vials, the collector, not the donor, removes the tamper-evident seals from the control form and places them on the specimen vials. Seal "A" goes over the primary vial containing 30 mL; seal "B" goes over the secondary vial containing 15 mL. (When doing a Non-Regulated drug screen, since only one vial would be used, "A" would be the appropriate tamper-evident seal to use.)

The seal must be centered over the lid and down the sides of the vial to ensure that the lid cannot be removed without destroying the seal.