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The page below is a sample from the LabCE course Department of Transportation (DOT) Federally Regulated Urine Specimen Collection Training. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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Pouring the Collected Specimen into the Specimen Bottle(s)

After the donor has handed his or her specimen to the collector, the collector now opens the specimen bottle(s). Make sure that the security seals for the specimen bottle(s) are only opened in the presence of the donor.

The collector, not the donor, pours the urine specimen into the specimen bottle(s).

Federally Regulated (DOT):

A minimum of 30 mL is poured into the primary specimen bottle (bottle A), a minimum of 15 mL is poured into the second specimen container (bottle B).

Non-Regulated:

A minimum of 30 mL is poured into just one of the specimen containers.

Note: The collector should not fill the primary or split specimen bottle up to the cap because a completely full bottle is more likely to leak in transit. Additionally, when a split specimen bottle is full and subsequently frozen, it may cause the bottle material to crack and then leak during transit as the specimen thaws.