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The page below is a sample from the LabCE course PCR Fundamentals: Focus on Multiplex PCR Assay and the Advantages over Singleplex Assays. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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Define Targets

A representative sample of both positive and negative samples must be selected to assess the analytical characteristics of the assay; for seeded samples, specimens must be incorporated into a suitable "matrix" to simulate the specimen.