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The page below is a sample from the LabCE course PCR Fundamentals: Focus on Multiplex PCR Assay and the Advantages over Singleplex Assays. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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Match the following assay validation parameter definitions to the parameters they define.

Select the correct match for each item from the drop-down box

Correlation in agreement between a series of measurements obtained from multiple sampling of the homogeneous sample performed under the exact assay conditions.
Ability of an assay to give a NEGATIVE result for patients not having the disease/test parameter in question.
Ability of an assay to give a POSITIVE result for patients having the disease/test parameter in question.
Correlation between the PCR results and KNOWN positive reference results.
Calculated nucleotide components to identify specific gene sequences.