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The page below is a sample from the LabCE course PCR Fundamentals: Focus on Multiplex PCR Assay and the Advantages over Singleplex Assays. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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Sample Transport and Processing Criteria

Like singleplex assays, attention must first be given to:
  • Sample collection: Assure a representative, validated sample is processed and ample nucleic acids are present.
  • Transport: Assure there is a system in place to maintain the stability of nucleic acids (eg, on ice or with RNA stabilized media when appropriate).
  • Storage: If sample is not processed immediately, storage occurs most commonly at -80°C.
  • Sample processing: Adhere to Standard Precautions.
  • Nucleic acid extraction: When applicable, use manual or automated extraction.
  • Contamination: Avoid contamination by having an appropriate environment that optimizes containment and adheres to post processing decontamination (e.g., ethanol and bleach).