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The page below is a sample from the LabCE course PCR Fundamentals: Focus on Multiplex PCR Assay and the Advantages over Singleplex Assays. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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You tested 150 (value for 'n') children in your pediatric clinic with your new Bordetella pertussis assay. Your test results showed:
50 (value for 'a') positive tests among 60 clinical cases of pertussis (60-50=10 [value for 'c']).
90 (value for 'd') patients without a cough (no pertussis) tested negative.
Determine the sensitivity, specificity, positive predictive value, and negative predictive value for the assay.

Select the correct match for each item from the drop-down box

Sensitivity %: a/(a+c) x 100
Specificity%: d/(d+b)x 100
Positive Predictive value %: a/(a+b) x 100
Negative Predictive value %: d/(d+c) x 100