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The page below is a sample from the LabCE course Clostridium difficile Infection (CDI): Overview, Laboratory Tests and Updated Guidelines.. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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The C. DIFF QUIK CHEK COMPLETEĀ® (continued)

The following is an overview of the C Diff Quik Chek Complete® test procedure:

  • The test is initiated by adding the stool sample to a tube containing a mixture of diluent and antibody-peroxidase conjugates.The diluted sample-conjugate mixture is added to the sample well within the device and the device is then allowed to incubate at room temperature for 15 minutes. During the incubation, any GDH antigen and toxins A and B in the sample will bind to the antibody-peroxidase conjugates.
  • The antigen-antibody-conjugate complexes migrate through a filter pad to a membrane where they are captured by the immobilized GDH-specific and toxins A and B-specific antibodies in the lines. The reaction window is subsequently washed with wash buffer, followed by the addition of substrate (tetramethylbenzidine) and allowed to incubate for 10 minutes.
  • After the 10-minute incubation period, the GDH antigen reaction is examined visually for the appearance of a vertical blue line on the “Ag line” of the reaction window. The toxins A & B reaction is visually examined for a vertical blue line on the “Toxin line” of the reaction window. A vertical dotted blue line on the “control line” confirms that the test is working properly and test result should be valid.
More test information follows.