The page below is a sample from the LabCE course Electrophoresis. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

Learn more about Electrophoresis (online CE course) »
How to Subscribe
MLS & MLT Comprehensive CE Package
Includes 97 CE courses, most popular
$95 Add to cart
Pick Your Courses
Up to 8 CE hours
$50 Add to cart
Individual course$20 Add to cart

Troubleshooting Irregular, Distorted or Atypical Bands

Band Appearance

Possible Cause

Short Migration Patterns

Contaminated or Aged Buffer

Diffuse Bands

Markedly Wet Gels
Poor Sample Application

Streaks Perpendicular to Bands

Tearing/Poking Gel in Sample Application

Weak Bands

Not Enough Sample
Not Enough Stain

Other Causes of Irregular and Distorted BandsHemolyzed Sample
Bent or Dirty Applicator
Air Bubbles in Sample Application
Too Much Sample
Wick Flow
Too Much Heat or Drying