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To prevent microbial growth and contamination, buffers must be refrigerated when not in use. Using a buffer at refrigerator temperature also improves band resolution and lessens evaporation during electrophoresis.

It is recommended that an electrophoresis using a small volume of buffer use a fresh buffer for each run. After a large volume buffer electrophoresis, the buffer can be reused if the buffer from each reservoir is combined, mixed well, and refrigerated.