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The page below is a sample from the LabCE course Detecting and Evaluating Coagulation Inhibitors and Factor Deficiencies. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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Mixing Study: Specimen Requirements

The specimen drawn for a mixing study must meet the following conditions for accurate testing:
  • A properly filled 3.2% sodium citrate tube (as shown in the image on the right) must be collected.
  • Proper centrifugation to create platelet-poor plasma for analysis must occur; the presence of platelet phospholipids can interfere with the mixing study if an anti-phospholipid antibody is present.
  • Testing must be performed within four hours of collection.