Immunoassay Techniques Used in Drug Test Screening: Enzyme Multiplied Immunoassay Technique (EMIT)

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Immunoassay Techniques Used in Drug Test Screening: Enzyme Multiplied Immunoassay Technique (EMIT)

Enzyme multiplied immunoassay technique (EMIT) is one of the most common drug screening platforms in use today. It was developed by the Syva Company in 1970. In this enzyme immunoassay (EIA), nicotinamide adenine dinucleotide (NAD) is reduced to NADH when it reacts with the enzyme G6PD. A change in absorbance is measured at 340 nm by a spectrometer.