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The page below is a sample from the LabCE course Fundamentals of Molecular Diagnostics (retired 2/12/2013). Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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As seen in the preceding table of amplified nucleic acid test methodologies, any of the following can be amplified:

  • Target (most common)
  • Probe
  • Signal

When employing an amplification procedure, each methodology can differ based on:

  • Amount of target
  • Amplification type
  • Enzyme requirements
  • Thermal cycling (thermocycling) requirements
  • Detection methodology