Home Products Most Popular Contact
No items in your cart.
The page below is a sample from the LabCE course Tissue Decalcification for Paraffin Processing. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

Learn more about Tissue Decalcification for Paraffin Processing (online CE course) »
How to Subscribe
Histology CE Package$65 Add to cart
Individual course$20 Add to cart

Mechanical Methods of End-point Determination

Although mechanical and physical methods are most commonly used in laboratories because they are quick and inexpensive, they are also the most destructive and invasive methods which introduce artifacts into tissue.
Mechanical method:
  • Tissue is tested by feeling for calcified bone by bending/flexing tissue
  • If the tissue is flexible, calcium is not present
  • Tissue can also be probed with a pin or needle in various locations
  • If pin inserts easily, calcium is not present
  • Artifacts can be introduced and tissue may be broken and damaged during testing
  • This method is not accurate at determining the end-point of decalcification and bone is frequently left in decalcifier for too long using this method