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The page below is a sample from the LabCE course HER2 Testing in Breast Cancer. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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Need for Hematoxylin and Eosin (H&E) Slide

This image is an H&E stained section. The tumor area in an H&E slide is marked for subsequent IHC and FISH testing.
It is critical that analysis be conducted on the invasive component of the breast cancer. This is because HER2 is, for unclear reasons, frequently increased (overexpressed and/or over amplified) in ductal carcinoma in situ (DCIS), and the clinical implications of this finding are uncertain.