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The page below is a sample from the LabCE course Breast Cancer Predictive Markers. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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Fluorochromes (fluorescent chromogens) are the molecules which are attached to the DNA probe that provide a method for visualization. Fluorophores on the DNA probe will fluoresce (give off) a specific color of light with exposure to certain wavelengths of light, which can be viewed only with a fluorescent microscope. When the light hits these molecules, it excites them and causes them to emit a complementary, longer wavelength of light at a lower energy level. The number and pattern of fluorescent signals observed can be used for a quantitative diagnostic evaluation of the breast tumor.