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The page below is a sample from the LabCE course Immunohistochemistry (IHC) Basics in Histology. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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System Quality Control (QC)

System QC is usually an antibody that has been tested and stains every time under the identical conditions of which the patient tissue has been subjected to.
An example of a good system control antibody is vimentin. Vimentin is the most commonly used antibody for a system control. Using a system control such as vimentin provides a visual account of the staining methodology (manual or an automated system) and whether or not it is working properly. One drawback to using a system quality control is the creation of extra slides:
  • One of a known positive tissue
  • One slide you incubate using the vimentin
  • Two slides for negative (one for negative control reagent and the other for negative tissue control)