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The page below is a sample from the LabCE course Immunohistochemistry (IHC) Basics in Histology. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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Blocking Nonspecific Background Staining

Nonspecific background staining can be attributed to endogenous avidin-biotin, to nonspecific antibody binding and to the presence of endogenous enzymes. To enhance staining, reduce nonspecific staining and avoid false-positive staining results, blocking methods are required in all IHC staining techniques. Blocking methods include those that utilize unlabeled avidin-biotin, serum and enzyme-quenching chemicals.