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The page below is a sample from the LabCE course Immunohistochemistry (IHC) Basics in Histology. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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Formalin/Aldehyde Substitutes

Formalin substitutes have been developed to fix tissue while avoiding the antigen masking effects of formalin. They can be aqueous-based and/or alcohol-based preparations. Due to the nature of these water or alcohol-based substitutes, penetration is slower than with formalin. Thus, fixation times need to be increased and tissue samples must be trimmed as small as possible to adequately fix the tissue. Some advantages to using these substitutes is that little or no antigen retrieval is necessary, they are less toxic (but still toxic), and they come pre-mixed and ready to use.
Caution must be used when using when using a formalin substitute. The additives in the fixative may give false-positives or false-negative results. Again, it is important to check the specification sheet for the antibody you are using.