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The page below is a sample from the LabCE course Real-Time PCR. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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Repetitive Cycling

The extension process takes approximately one minute at which time the whole process can start over again. Repetitive cycling results in the exponential accumulation of product. In most cases, 20 to 40 cycles are required to be detected by whatever method is employed. Since each complete cycle only takes seconds to minutes, the whole process can be completed within an hour. In conventional PCR methods, this is the point at which the synthesized DNA can be analyzed.