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Enzyme Cofactors, dNTPs, and Salts/Buffers

It is important to have extra dNTPs and magnesium so the reaction does not run out of components.
  • dNTPs are the nucleotide bases added to the growing DNA strand by the DNA polymerase.
  • The concentration of MgCl2 influences the stringency of the interaction between the primers and the template DNA.
Salt and buffers
  • Stabilize enzyme function and formation of primer template hybrids. Buffers often contain Tris HCl, KCl, and sometimes MgCl2.