Home Products Most Popular Contact
No items in your cart.
The page below is a sample from the LabCE course Real-Time PCR. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

Learn more about Real-Time PCR (online CE course) »
How to Subscribe
MLS & MLT Comprehensive CE Package
Includes 111 CE courses, most popular
$95 Add to cart
Pick Your Courses
Up to 8 CE hours
$50 Add to cart
Individual course$20 Add to cart

Enzyme Cofactors, dNTPs, and Salts/Buffers

It is important to have extra dNTPs and magnesium so the reaction does not run out of components.
dNTPS
  • dNTPs are the nucleotide bases added to the growing DNA strand by the DNA polymerase.
MgCl2
  • The concentration of MgCl2 influences the stringency of the interaction between the primers and the template DNA.
Salt and buffers
  • Stabilize enzyme function and formation of primer template hybrids. Buffers often contain Tris HCl, KCl, and sometimes MgCl2.