Picking Selected Panel Cells Conservatively - LabCE.com, Laboratory Continuing Education

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The page below is a sample from the LabCE course Antibody Detection and Identification. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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Picking Selected Panel Cells Conservatively

Choose cells that can help rule out more than one antibody at a time in order to help decrease supply usage and prep time.

Example: You suspect a patient has developed anti-Jk^a and you'd like to be as efficient as possible in both time and utilization of resources. You have ruled out all other antigens except for C, Fy^b, and M. To optimize your process, selecting cell 9 which contains homozygous expressions of each emerges as the most suitable choice.

	Rh-Hr		Duffy		Kidd		MNSs
Panel Cell	C	c	Fy^a	Fy^b	Jk^a	Jk^b	M	N
9	+	0	0	+	0	+	+	0
10	+	0	+	+	0	+	0	+
11	+	+	0	+	0	+	+	0
12	+	+	+	+	0	+	+	0

Instead of running 3 separate cells to rule out the antibodies, you can choose one that is homozygous positive for C, Fy^b, and M, and negative for Jk^a. Panel cell 9 works in this case.

If the only antibody that is present is anti-Jk^a, then your test results should be negative. If the results are positive then further rule-outs will be needed to determine what is present.