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The page below is a sample from the LabCE course Rh-Negative Female with Anti-D at Delivery: A Case Study. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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Screening for Fetomaternal Hemorrhage (FMH)

FMH greater than 30 mL of whole blood occurs in only about 0.3% of cases but must be detected to prevent the mother from producing anti-D. Once the mother has become immunized, it cannot be undone and RhIG is of no use.
A typical test protocol is first to screen for a large FMH and then quantitate the bleed if the screen is positive. Some laboratories proceed directly to a test that can quantitate the size of the FMH.

Once the size of the FMH is determined, a formula is used to determine how much RhIG is needed. Recall that:
  • A standard vial of RhIG contains 1500 IU (300 µg) of IgG anti-D;
  • 300 µg of RhIG can suppress immunization to approximately 30 mL of D-positive whole blood.
Several methods are available to detect an FMH that requires additional RhIG.
Acceptable screening tests for FMH include
  • Rosette method;
  • Commercial fetal bleed screening tests;
  • Gel agglutination fetal cell screening technique.
Note: The rosette test may be falsely positive if the mother is weak-D positive and may be falsely negative if the baby is weak-D positive.