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The page below is a sample from the LabCE course Theoretical and Practical Aspects of Routine H&E Staining. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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Paraffin Sections

The following steps are performed after sectioning tissue samples that have been processed in the routine manner (formalin-fixed paraffin-embedded) and placing them on the glass microscopic slides:
1. Deparaffinization (usually a two step process):
  • First, the slides are placed in a drying oven to remove the water from the tissue, help adhere the section to the slide, and start removing the wax from around the tissue.
  • Then, the slides are brought through 2-3 changes of a solvent such as xylene (xylene substitute may also be used) which removes the remaining paraffin from the slides and tissue so that the dyes can then penetrate into the cells.
2. Hydration: This is accomplished by using a series of graded alcohols to reintroduce water to the tissue.
  • Hydration begins as the slides go through absolute alcohol which removes the solvent/agent used for deparaffinization.
  • Slides then go through several changes of alcohols that are diluted in concentration until they reach water. This is a necessary process which prepares the tissue to stain with the aqueous staining solution.